脂多糖诱导的自身抗体反应。II. 对菠萝蛋白酶处理的同基因红细胞的空斑形成细胞反应的年龄相关变化。

Lipopolysaccharide-induced autoantibody response. II. Age-related change in plaque-forming cell response to bromelain-treated syngeneic erythrocytes.

作者信息

Fujiwara M, Kariyone A

出版信息

Int Arch Allergy Appl Immunol. 1981;66(2):161-72.

Abstract

In spleen of normal mice, there are relatively large numbers of plaque-forming cells (PFC) against syngeneic or allogeneic red blood cells treated with bromelain (Br. MRBC) and the numbers of PFC remarkably increase by the injection of bacterial lipopolysaccharide (LPS). In this report, age-related change in the anti-Br. MRBC PFC response was studied in vivo and in vitro. It was revealed that mean numbers of splenic anti-Br. MRBC PFC increased in both untreated and LPS-injected mice with age (3--14 months). The increase in anti-Br.MRBC PFC detected in LPS-stimulated spleen was dependent on the numbers of PFC in untreated spleen, but not related to the numbers of anti-TNP PFC nor the degree of 3H-thymidine incorporation assessed in in vitro culture. These results imply that enhanced anti-Br.MRBC PFC response was not merely due to polyclonal activation of B lymphocytes. Reasons for increased PFC numbers were analyzed in special references to suppressor activities. The experimental results were obtained suggesting that suppressors were not involved in anti-Br.MRBC PFC response. There were also no marked differences in the acceptability of suppressive signals between young and older spleen cells. It was concluded that hightened anti-Br.MRBC PFC response in older mice might be due to spontaneous increase in the size of Br.MRBC-reactive clone.

摘要

在正常小鼠的脾脏中，存在相对大量针对经菠萝蛋白酶处理的同基因或异基因红细胞（菠萝蛋白酶处理的红细胞）的噬斑形成细胞（PFC），并且通过注射细菌脂多糖（LPS），PFC的数量会显著增加。在本报告中，研究了体内和体外抗菠萝蛋白酶处理的红细胞PFC反应的年龄相关变化。结果显示，在未处理和注射LPS的小鼠中，脾脏抗菠萝蛋白酶处理的红细胞PFC的平均数量均随年龄（3 - 14个月）增加。在LPS刺激的脾脏中检测到的抗菠萝蛋白酶处理的红细胞PFC的增加依赖于未处理脾脏中PFC的数量，但与抗TNP PFC的数量或体外培养中评估的3H - 胸苷掺入程度无关。这些结果表明，抗菠萝蛋白酶处理的红细胞PFC反应增强不仅仅是由于B淋巴细胞的多克隆激活。特别参考抑制活性分析了PFC数量增加的原因。获得的实验结果表明，抑制因子不参与抗菠萝蛋白酶处理的红细胞PFC反应。年轻和老年脾细胞在接受抑制信号方面也没有明显差异。得出的结论是，老年小鼠中抗菠萝蛋白酶处理的红细胞PFC反应增强可能是由于对菠萝蛋白酶处理的红细胞有反应的克隆大小自发增加。