Pattus F, Rothen C, Streit M, Zahler P
Biochim Biophys Acta. 1981 Sep 21;647(1):29-39. doi: 10.1016/0005-2736(81)90292-3.
Air/water interface films were obtained from human erythrocytes and rabbit sarcoplasmic reticulum membranes at 'zero surface pressure. according to Verger, R and Pattus, F. (Chem. Phys. Lipids (1976) 16, 285-291). The lipid and protein distribution of these membrane films suggest that the film composition is determined by the composition of the membrane and the mode of integration of its components. When kept at low surface pressure, slow film expansion occurred due to unfolding of proteins at the interface. This process can be stopped by compressing the films at a higher surface pressure than 15 dyn/cm. Acetylcholinesterase activity from human erythrocyte films is highly dependent on the condensation state of the film. Ca2+-ATPase from sarcoplasmic reticulum films was still activable by Ca2+. Freeze-fracture studies on erythrocyte membrane films suggest the such films are monolayers in which proteins are randomly distributed.
根据韦尔热（Verger，R）和帕图斯（Pattus，F）（《化学与物理脂质》（1976年）第16卷，第285 - 291页）的方法，在“零表面压力”下从人红细胞和兔肌质网膜获得气/水界面膜。这些膜的脂质和蛋白质分布表明，膜的组成由膜的组成及其成分的整合方式决定。当保持在低表面压力时，由于界面处蛋白质的展开，膜会缓慢膨胀。通过在高于15达因/厘米的表面压力下压缩膜，可以阻止这一过程。人红细胞膜的乙酰胆碱酯酶活性高度依赖于膜的凝聚状态。肌质网膜的Ca2 + - ATP酶仍可被Ca2 +激活。对红细胞膜的冷冻蚀刻研究表明，这种膜是蛋白质随机分布的单层膜。
