Langhorne J, Evans C B, Asofsky R, Taylor D W
Cell Immunol. 1984 Sep;87(2):452-61. doi: 10.1016/0008-8749(84)90014-5.
The antibody response of mice to Plasmodium chabaudi adami and Plasmodium yoelii has been compared using a solid phase isotype-specific radioimmunoassay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Serological cross-reactivity between these parasites was substantial. Studies using a radioimmunoassay detecting all classes of malaria-specific antibody demonstrated that during the early part of infection it was not possible to distinguish between homologous and heterologous reactions. Immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that 50% or more of the protein antigens detected were apparently shared by both parasites although the intensity of bands was always greater with homologous reactions. However, the distribution of isotypes in the antibody (Ab) response differed in the two infections. P. chabaudi infections were characterized by a predominant and persistent IgM response, moderate IgG2 and IgG3 and little significant IgG1 response during a primary infection. By contrast, IgM antibodies were transient in P. yoelii infection, IgG2 was the predominant isotype, and both IgG1 and IgG3 antibodies were present during a primary infection. These differences in isotypes were also detected when sera were tested on the heterologous antigen extracts suggesting that antigens shared by P. chabaudi and P. yoelii do not necessarily induce similar antibody responses in the two infections.
已使用固相同种型特异性放射免疫测定法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，比较了小鼠对恰氏疟原虫和约氏疟原虫的抗体反应。这些寄生虫之间的血清学交叉反应很显著。使用检测所有类别的疟疾特异性抗体的放射免疫测定法进行的研究表明，在感染初期，无法区分同源反应和异源反应。免疫沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，尽管同源反应的条带强度总是更高，但检测到的50%或更多的蛋白质抗原显然为两种寄生虫所共有。然而，两种感染中抗体（Ab）反应的同种型分布有所不同。恰氏疟原虫感染的特征是在初次感染期间主要为持续的IgM反应、中度的IgG2和IgG3反应，以及几乎没有显著的IgG1反应。相比之下，在约氏疟原虫感染中IgM抗体是短暂的，IgG2是主要的同种型，并且在初次感染期间IgG1和IgG3抗体均存在。当用异源抗原提取物检测血清时，也检测到了这些同种型差异，这表明恰氏疟原虫和约氏疟原虫共有的抗原不一定在两种感染中诱导相似的抗体反应。
