Tsuruo T, Naganuma K, Yamori T, Kawabata H, Oh-Hara T, Iida H, Tsukagoshi S, Sakurai Y
Gan. 1984 Jun;75(6):557-63.
Spontaneous metastasis of highly metastatic variants, B16 melanoma BL-6 and colon adenocarcinoma 26 NL-22, was examined. Tumor cells were inoculated into the right front footpad and the original tumors were removed by amputation of the forelimb at an appropriate time after tumor inoculation. Spontaneous lymph node and lung metastases occurred with B16 BL-6 and spontaneous lung metastasis occurred with NL-22. Analysis of the time-dependent formation of lymph node and lung metastases of BL-6 indicated that lung metastasis could be formed after the lymph node metastasis. Metastasis is influenced by the mouse strain, and both inbred and F1 hybrid mice of syngeneic origin could be used as host animals. By using these metastatic variants, two spontaneous metastasis systems were established. In order to evaluate the systems, the effects of 5-fluorouracil (5-FU) and adriamycin (ADM) were examined. 5-FU was effective against the metastases of both tumors, and ADM was moderately effective against B16 BL-6 metastasis.
对高转移性变体B16黑色素瘤BL-6和结肠腺癌26 NL-22的自发转移进行了研究。将肿瘤细胞接种到右前足垫，并在接种肿瘤后的适当时间通过截肢前肢切除原发肿瘤。B16 BL-6出现了自发的淋巴结和肺转移，NL-22出现了自发的肺转移。对BL-6淋巴结和肺转移的时间依赖性形成分析表明，肺转移可在淋巴结转移后形成。转移受小鼠品系的影响，同基因来源的近交系和F1杂交小鼠均可作为宿主动物。通过使用这些转移变体，建立了两个自发转移系统。为了评估这些系统，检测了5-氟尿嘧啶（5-FU）和阿霉素（ADM）的作用。5-FU对两种肿瘤的转移均有效，ADM对B16 BL-6转移有中度疗效。
