Kainate reversibly aggregates brain tubulin in vitro.
作者信息
López-Colomé A M, Casas A
出版信息
Biochem Biophys Res Commun. 1984 Aug 16;122(3):925-31. doi: 10.1016/0006-291x(84)91179-3.
PMID:6477571
Abstract
The effect of kainate, an heterocyclic analogue of glutamate on tubulin polymerization was studied. We demonstrate that kainate induces a dose-dependent aggregation of rat brain tubulin either purified by DEAE-Sephadex A-50 or in the presence of MAPs into a mesh-like structure. Such polymer is cold-, CaCl2- and colchicine-insensitive. Removal of kainate from the incubation medium yields free tubulin competent for polymerizing into normal microtubules in the presence of GTP.
摘要
相似文献
1
Kainate reversibly aggregates brain tubulin in vitro.
Biochem Biophys Res Commun. 1984 Aug 16;122(3):925-31. doi: 10.1016/0006-291x(84)91179-3.
2
Quantification and properties of tubulin polymerization in crude brain extracts and preparations of microtubular and purified tubulin.
Eur J Biochem. 1978 Nov 2;91(1):151-61. doi: 10.1111/j.1432-1033.1978.tb20947.x.
3
In vitro assembly of rat pancreas tubulin in the presence of taxol.
Biochim Biophys Acta. 1986 May 12;871(1):72-7. doi: 10.1016/0167-4838(86)90134-2.
4
Mechanism of colchicine-dimer addition to microtubule ends: implications for the microtubule polymerization mechanism.
Biochemistry. 1980 Nov 25;19(24):5550-7. doi: 10.1021/bi00565a014.
5
Tubulin-colchicine interactions and polymerization of the complex.
Ann N Y Acad Sci. 1986;466:676-89. doi: 10.1111/j.1749-6632.1986.tb38451.x.
6
Characterization and in vitro polymerization of Tetrahymena tubulin.
J Biochem. 1978 Apr;83(4):1065-75. doi: 10.1093/oxfordjournals.jbchem.a131995.
7
Sulfhydryl groups of Mimosa pudica tubulin implicated in colchicine binding and polymerization in vitro.
Arch Biochem Biophys. 1992 May 1;294(2):353-60. doi: 10.1016/0003-9861(92)90695-s.
8
Role of tubulin-SH groups in polymerization to microtubules. Functional-SH groups in tubulin for polymerization.
J Biochem. 1974 Sep;76(3):651-4. doi: 10.1093/oxfordjournals.jbchem.a130609.
9
Tubulin bound to colchicine forms polymers different from microtubules.
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6753-6. doi: 10.1073/pnas.79.22.6753.
10
Interaction of cryptophycin 1 with tubulin and microtubules.
FEBS Lett. 1995 Dec 11;377(1):59-61. doi: 10.1016/0014-5793(95)01271-0.
Zotero 插件