D'Amore P A, Klagsbrun M
J Cell Biol. 1984 Oct;99(4 Pt 1):1545-9. doi: 10.1083/jcb.99.4.1545.
Bovine retina and hypothalamus contain anionic endothelial cell mitogens that display unusual affinities for the negatively charged glycosaminoglycan heparin. Both growth factor activities are acidic polypeptides (pl's of 5.0) as determined by isoelectric focusing and DEAE-affinity chromatography. In spite of their anionic nature, the factors bound to heparin-Sepharose columns with high affinity and could be eluted only at high salt concentrations (0.9-1.1 M NaCl). The affinity of the retina-derived growth factor (RDGF) for heparin permitted a 15,000-fold purification of the mitogen in two steps: heparin-affinity chromatography and size exclusion high-performance liquid chromatography. RDGF and the anionic hypothalamus-derived factor (aHDGF) exhibit three major biochemical similarities including isoelectric point, (pl's of 5.0), heparin affinity (elution at 0.9-1.1 M NaCl) and molecular weight (18,000). Additionally, the two factors display similar biological activities, stimulating the proliferation of capillary and human umbilical vein endothelial and 3T3 cells but not vascular smooth muscle cells. We suggest that RDGF and aHDGF are related if not identical growth factor molecules.
牛视网膜和下丘脑含有阴离子型内皮细胞促细胞分裂剂，这些促细胞分裂剂对带负电荷的糖胺聚糖肝素表现出不同寻常的亲和力。通过等电聚焦和DEAE-亲和色谱法测定，这两种生长因子活性均为酸性多肽（等电点为5.0）。尽管它们具有阴离子性质，但这些因子与肝素-琼脂糖柱具有高亲和力，并且仅在高盐浓度（0.9 - 1.1M NaCl）下才能洗脱。视网膜衍生生长因子（RDGF）对肝素的亲和力使得在两步中能将促细胞分裂剂纯化15000倍：肝素亲和色谱法和尺寸排阻高效液相色谱法。RDGF和阴离子型下丘脑衍生因子（aHDGF）表现出三个主要的生化相似性，包括等电点（5.0）、肝素亲和力（在0.9 - 1.1M NaCl下洗脱）和分子量（18000）。此外，这两种因子表现出相似的生物学活性，刺激毛细血管、人脐静脉内皮细胞和3T3细胞的增殖，但不刺激血管平滑肌细胞。我们认为RDGF和aHDGF即使不是完全相同的生长因子分子，也是相关的。
