Ziskind-Conhaim L, Geffen I, Hall Z W
J Neurosci. 1984 Sep;4(9):2346-9. doi: 10.1523/JNEUROSCI.04-09-02346.1984.
The mechanism of formation of acetylcholine receptor (AChR) clusters at developing mammalian endplates was investigated in vitro, using intercostal muscles from embryonic rats. The muscles were explanted in organ culture with the spinal cord attached, as described previously (Ziskind-Conhaim, L., and M. J. Dennis (1981) Dev. Biol. 85: 243-251). AChRs on the myofibers were labeled with [125I]-alpha-bungarotoxin shortly before clusters appeared and subsequently were cultured in unlabeled toxin for 1 day. Autoradiography of the cultured fibers demonstrated the presence of labeled clusters of AChRs indicating that the AChRs in the newly formed clusters arise from AChRs that had previously been uniformly distributed on the muscle surface.
利用来自胚胎大鼠的肋间肌,在体外研究了发育中的哺乳动物终板处乙酰胆碱受体(AChR)簇的形成机制。如先前所述(Ziskind-Conhaim, L., and M. J. Dennis (1981) Dev. Biol. 85: 243-251),将肌肉连同附着的脊髓一起移植到器官培养物中。在簇出现前不久,用[125I]-α-银环蛇毒素标记肌纤维上的AChR,随后在未标记的毒素中培养1天。对培养的纤维进行放射自显影,结果显示存在标记的AChR簇,这表明新形成的簇中的AChR来自先前均匀分布在肌肉表面的AChR。