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[用于电子显微镜研究准备的鼠疫感染跳蚤消毒方法]

[Method of disinfecting plague-infected fleas in preparation for electron microscopic study].

作者信息

Shchedrin V I, Vashchenok V S, Shashaev M A, Briukhanov L V, Osipova S P

出版信息

Parazitologiia. 1984 Jul-Aug;18(4):317-8.

PMID:6483471
Abstract

During fixation of fleas infected with plague agent (with preliminary cut heads, limbs and posterior part of the abdomen) in 2.5% glutaric dialdehyde or 2% osmium tetroxide their disinfection was obtained in two days. After double fixation with 2-hour exposure in each of these fixators disinfection of the material was acheived only after additional 2-day maintenance in 70% ethyl alcohol. Fleas, which had been placed into fixators or in 70% ethyl alcohol after double fixation without preliminary dissection, were disinfected completely only in 10 days.

摘要

在用2.5%戊二醛或2%四氧化锇固定感染鼠疫杆菌的跳蚤(预先切除头部、肢体和腹部后部)时,两天后可实现消毒。在用这些固定剂分别进行2小时的双重固定后,材料仅在70%乙醇中额外保存2天后才实现消毒。在未预先解剖的情况下进行双重固定后放入固定剂或70%乙醇中的跳蚤,仅在10天后才完全消毒。

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