胆固醇与兔脑突触体细胞膜结合的温度效应
Temperature effect of cholesterol association with synaptosomal plasma membranes of rabbit brain.
作者信息
Deliconstantinos G
出版信息
Biochem J. 1984 Sep 15;222(3):825-8. doi: 10.1042/bj2220825.
Abstract
Association of exogenous cholesterol with rabbit brain synaptosomal plasma membranes follows an exponential path described by the general formula y = a X ebx. The co-operative nature of this association was shown when increasing amounts of unlabelled cholesterol glucoside (up to 0.5 mM) were added to a fixed amount (5 microM) of [14C]cholesterol, when a biphasic curve of the binding of [14C]cholesterol into the membranes was obtained. Arrhenius plots of this association revealed two break points which occur at 25 degrees C and 42 degrees C. The first break apparently corresponds to the transition from the crystalline to the gel phase. The second break may be due to the (continuously) increasing entropy of the system which creates at a certain point difficulties in the binding of cholesterol into the lipid bilayer.
摘要
外源性胆固醇与兔脑突触体细胞膜的结合遵循由通式y = a X ebx描述的指数路径。当向固定量（5 microM）的[14C]胆固醇中添加逐渐增加量的未标记胆固醇葡糖苷（高达0.5 mM）时，获得了[14C]胆固醇与膜结合的双相曲线，表明了这种结合的协同性质。这种结合的阿伦尼乌斯图显示出两个断点，分别出现在25℃和42℃。第一个断点显然对应于从晶相到凝胶相的转变。第二个断点可能是由于系统熵（持续）增加，在某一点上导致胆固醇与脂质双层结合出现困难。
相似文献
1
Temperature effect of cholesterol association with synaptosomal plasma membranes of rabbit brain.胆固醇与兔脑突触体细胞膜结合的温度效应
Biochem J. 1984 Sep 15;222(3):825-8. doi: 10.1042/bj2220825.
2
Evoked effects of cholesterol binding on integral proteins and lipid fluidity of dog brain synaptosomal plasma membranes.胆固醇结合对犬脑突触体细胞膜整合蛋白和脂质流动性的诱发效应。
Biochem Cell Biol. 1989 Jan;67(1):16-24. doi: 10.1139/o89-003.
3
Structure activity relationship of cholesterol and steroid hormones with respect to their effects on the Ca2+-stimulated ATPase and lipid fluidity of synaptosomal plasma membranes from dog and rabbit brain.胆固醇和类固醇激素对犬及兔脑突触体细胞膜上钙刺激的ATP酶及脂质流动性影响的构效关系
Comp Biochem Physiol B. 1988;89(3):585-94. doi: 10.1016/0305-0491(88)90178-2.
4
Calmodulin selectively modulates the guanylate cyclase activity by repressing the lipid phase separation temperature in the inner half of the bilayer of rat brain synaptosomal plasma membranes.
Neurochem Res. 1989 Feb;14(2):119-27. doi: 10.1007/BF00969626.
5
Effect of rat serum albumin-cholesterol on the physical properties of biomembranes.大鼠血清白蛋白-胆固醇对生物膜物理性质的影响。
Biochem Int. 1987 Aug;15(2):467-74.
6
[Exocytotic steps in cell-free system after cholesterol deprivation in synaptosomal plasma membranes and synaptic vesicles].[突触体细胞膜和突触小泡胆固醇剥夺后无细胞体系中的胞吐步骤]
Ukr Biokhim Zh (1999). 2011 Mar-Apr;83(2):53-64.
7
Changes in the form of Arrhenius plots of the activity of glucagon-stimulated adenylate cyclase and other hamster liver plasma-membrane enzymes occurring on hibernation.冬眠时仓鼠肝脏质膜中胰高血糖素刺激的腺苷酸环化酶及其他酶活性的阿伦尼乌斯曲线形式的变化。
Biochem J. 1978 Sep 15;174(3):909-19. doi: 10.1042/bj1740909.
8
Cooperative nature of the binding of cholesterol on to synaptosomal plasma membranes of dog brain.胆固醇与犬脑突触体细胞膜结合的协同性质。
Biochim Biophys Acta. 1981 May 20;643(3):642-9. doi: 10.1016/0005-2736(81)90360-6.
9
Effects of aging and beta-amyloid on the properties of brain synaptic and mitochondrial membranes.衰老和β-淀粉样蛋白对脑突触膜和线粒体膜特性的影响。
J Neural Transm (Vienna). 2001;108(8-9):1051-64. doi: 10.1007/s007020170024.
10
引用本文的文献
1
Increase of particulate nitric oxide synthase activity and peroxynitrite synthesis in UVB-irradiated keratinocyte membranes.紫外线B照射的角质形成细胞膜中颗粒性一氧化氮合酶活性和过氧亚硝酸盐合成增加。
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):997-1003. doi: 10.1042/bj3200997.
2
Differential effect of anionic and cationic drugs on the synaptosome-associated acetylcholinesterase activity of dog brain.阴离子和阳离子药物对犬脑突触体相关乙酰胆碱酯酶活性的差异影响。
Biochem J. 1985 Jul 1;229(1):81-6. doi: 10.1042/bj2290081.
3
Carbon tetrachloride modulates the rat hepatic microsomal UDP-glucuronyl transferase activity and membrane fluidity.四氯化碳可调节大鼠肝脏微粒体UDP-葡萄糖醛酸基转移酶活性及膜流动性。
Experientia. 1986 Feb 15;42(2):181-3. doi: 10.1007/BF01952457.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
4
Rates of cholesterol exchange between human erythrocytes and plasma lipoproteins.人体红细胞与血浆脂蛋白之间的胆固醇交换速率。
Biochim Biophys Acta. 1980 Aug 4;600(2):530-41. doi: 10.1016/0005-2736(80)90454-x.
5
Cooperative nature of the binding of cholesterol on to synaptosomal plasma membranes of dog brain.胆固醇与犬脑突触体细胞膜结合的协同性质。
Biochim Biophys Acta. 1981 May 20;643(3):642-9. doi: 10.1016/0005-2736(81)90360-6.
6
Modulation of serotonergic receptors by exogenous cholesterol in the dog synaptosomal plasma membrane.外源性胆固醇对犬突触体细胞膜中血清素能受体的调节作用。
Biochem Pharmacol. 1980 Dec;29(24):3325-7. doi: 10.1016/0006-2952(80)90311-1.
7
Free cholesterol not carried by lipoproteins in human serum.人血清中未被脂蛋白携带的游离胆固醇。
Experientia. 1983 Jul 15;39(7):748-50. doi: 10.1007/BF01990306.
8
Specificity of binding of cholesterol, steroid hormones and other compounds in synaptosomal plasma membranes, and their effect on ouabain-sensitive ATPase.突触体细胞膜中胆固醇、类固醇激素及其他化合物的结合特异性及其对哇巴因敏感的ATP酶的影响。
Biochim Biophys Acta. 1981 May 20;643(3):650-8. doi: 10.1016/0005-2736(81)90361-8.
9
Influence of phosphatidylserine on (Na+ + K+)-stimulated ATPase and acetylcholinesterase activities of dog brain synaptosomal plasma membranes.磷脂酰丝氨酸对犬脑突触体细胞膜（Na+ + K+）刺激的ATP酶和乙酰胆碱酯酶活性的影响。
Biochem J. 1984 May 15;220(1):301-7. doi: 10.1042/bj2200301.
10
Alterations in the activities of hepatic plasma-membrane and microsomal enzymes during liver regeneration.肝脏再生过程中肝细胞膜和微粒体酶活性的变化。
Biochem J. 1983 May 15;212(2):445-52. doi: 10.1042/bj2120445.
Zotero 插件