Almeida A F, Jarvis S M, Young J D, Paterson A R
FEBS Lett. 1984 Oct 29;176(2):444-8. doi: 10.1016/0014-5793(84)81215-6.
Nitrobenzylthioniosine (NBMPR), a potent and specific inhibitor of nucleoside transport, is bound reversibly by high affinity sites on nucleoside transporter proteins of erythrocyte membranes and, upon photoactivation, NBMPR molecules become covalently bonded to the sites. This study showed that [3H]NBMPR molecules reversibly bound to intact S49 and L5178Y mouse lymphoma cells became covalently bound upon exposure to UV light. Electrophoretic analysis of plasma membrane fractions from the labelled cells showed that 3H was present in polypeptides which migrated as a major band with an apparent Mr of 45000-65000.
硝基苄基硫代肌苷（NBMPR）是一种有效的核苷转运特异性抑制剂，它通过红细胞膜核苷转运蛋白上的高亲和力位点可逆性结合，并且在光激活后，NBMPR分子会与这些位点共价结合。本研究表明，与完整的S49和L5178Y小鼠淋巴瘤细胞可逆性结合的[3H]NBMPR分子在暴露于紫外光后会共价结合。对标记细胞的质膜部分进行电泳分析显示，3H存在于迁移为主要条带的多肽中，其表观分子量为45000 - 65000。
