Chelidze P V, Larioni L K, Agladze A G, Dzhindzholiia Sh R
Tsitologiia. 1984 Aug;26(8):878-85.
The transformation of nucleolus and its structural components in the main groups of erythroid cells (from pronormoblasts to reticulocytes and dividing ones) has been studied. It is shown that during inactivation of the nucleolus, the granular component is reduced, and the degree of chromatin condensation increases. Enlargement and "naking" of fibrillar centres are also observed. At the stage of basophilic and polychromatophilic erythroblasts, the nucleolus has a mushroom-like shape with well developed fibrillar centres, which lie at the border of the nucleolus. Nucleolar RNP components consist predominantly of a fibrillar component and forms "caps" of these mushroom-like structures. Therefore, at this stage "free" fibrillar centres are found on ultrathin sections, if the section plane runs only through the fibrillar centre, or through ring-shaped nucleoli, i.e. the fibrillar centre surrounded by sheet of nucleolar RNP fibrilles, when the mushroom-like nucleolus is cut tangentially. Using serial section technique, small round nucleoli with an extremely weakly developed RNP material or free fibrillar centres, resembling those in telophase nuclei, are shown on the terminal stage of nucleolus transformation. It is noted that the main groups of erythroid cells differ from each other not only in the chromatin condensation degree, but also in the development of nucleolus material and in the size of fibrillar centres. However, such differences exist in either cell group. Consequently, we can distinguish between cell populations being on different stages of maturation. On this basis, we described on intermediate population of cells, which possess signs of pronormoblasts and basophilic erythroblasts. In all the cases, strands of electron opaque material bound with the condensed chromatin are present in fibrillar centres.(ABSTRACT TRUNCATED AT 250 WORDS)
对主要类别的红细胞（从原成红细胞到网织红细胞以及正在分裂的红细胞）中核仁及其结构成分的转变进行了研究。结果表明，在核仁失活期间，颗粒成分减少，染色质凝聚程度增加。还观察到纤维中心的增大和“裸露”。在嗜碱性和多色性成红细胞阶段，核仁呈蘑菇状，纤维中心发育良好，位于核仁边界。核仁核糖核蛋白成分主要由纤维成分组成，并形成这些蘑菇状结构的“帽”。因此，在这个阶段，如果切片平面仅穿过纤维中心，或者穿过环形核仁（即当蘑菇状核仁被切成切线时，由核仁核糖核蛋白纤维片包围的纤维中心），在超薄切片上会发现“游离”的纤维中心。使用连续切片技术，在核仁转变的末期显示出小圆形核仁，其核糖核蛋白物质发育极弱或有游离纤维中心，类似于末期细胞核中的情况。值得注意的是，主要类别的红细胞不仅在染色质凝聚程度上彼此不同，而且在核仁物质的发育和纤维中心的大小上也不同。然而，这些差异在任何一个细胞群体中都存在。因此，我们可以区分处于不同成熟阶段的细胞群体。在此基础上，我们描述了一个中间细胞群体，其具有原成红细胞和嗜碱性成红细胞的特征。在所有情况下，与凝聚染色质结合的电子不透明物质链存在于纤维中心。（摘要截短于250字）
