Laing N G
J Embryol Exp Morphol. 1984 Oct;83:213-23.
Chick embryo wing buds were rotated close to the lateral edge of the somites at stage 19, prior to limb innervation. Despite the abnormal orientation of the resulting limb, the motor pools to biceps and triceps were largely normal, as judged by electrical stimulation and horseradish peroxidase labelling just prior to hatching. The only abnormalities were a few caudal motoneurons innervating biceps and a few rostral motoneurons innervating triceps. This distribution is similar to that seen normally in young embryos before the completion of motoneurons death and it is suggested that the rotation may be keeping alive motoneurons which otherwise would die. The morphology of the brachial plexus supplying rotated wings was abnormal. It is concluded that axons growing into the limb bud from the spinal cord can compensate for reversal of both the limb axes and selectively innervate appropriate muscles. The result is consistent with others in which proximal reversal of one limb axis alone produced normal innervation.
在第19阶段，即在肢体神经支配之前，将鸡胚翼芽旋转至靠近体节的外侧边缘。尽管由此产生的肢体方向异常，但通过孵化前的电刺激和辣根过氧化物酶标记判断，肱二头肌和肱三头肌的运动神经元池基本正常。唯一的异常是少数支配肱二头肌的尾侧运动神经元和少数支配肱三头肌的头侧运动神经元。这种分布类似于在运动神经元死亡完成之前的年轻胚胎中正常看到的分布，并且有人认为这种旋转可能使原本会死亡的运动神经元存活下来。供应旋转翼的臂丛神经形态异常。得出的结论是，从脊髓长入肢体芽的轴突可以补偿肢体轴的反转，并选择性地支配适当的肌肉。这一结果与其他仅一个肢体轴近端反转产生正常神经支配的结果一致。
