针对人乳腺癌细胞系膜成分的单克隆抗体HBCA - 12所检测的靶抗原的生化和组织化学特征

Biochemical and histochemical characteristics of target antigen detected by monoclonal antibody HBCA-12 against a membrane component of human mammary carcinoma cell line.

作者信息

Kovarík J, Chorváth B, Duraj J, Bártek J, Rejthar A, Lauerová L, Babusíková O

出版信息

Neoplasma. 1984;31(6):625-9.

Abstract

Monoclonal antibody HBCA-12 obtained by hybridoma procedure after immunization with human mammary adenocarcinoma cell line MDA-MB-231 immunoprecipitated a cell surface sialoglycoprotein gp80 (apparent molecular weight 80 000) from MDA-MB-231 cells and a glycoprotein gp78 from human myeloma cell line ARH 77. A protein of a similar electrophoretic mobility was immunoprecipitated also from 35S-methionine metabolically radiolabeled human melanoma cell line VUP 1. The expression of the antigen recognized by HBCA-12 monoclonal antibody could be detected neither on PHA-induced nor on EBV-transformed peripheral blood mononuclear cells from healthy donors.

摘要

用人类乳腺腺癌细胞系MDA-MB-231免疫后通过杂交瘤技术获得的单克隆抗体HBCA-12，从MDA-MB-231细胞中免疫沉淀出一种细胞表面唾液酸糖蛋白gp80（表观分子量80000），并从人类骨髓瘤细胞系ARH 77中免疫沉淀出一种糖蛋白gp78。还从经35S-甲硫氨酸代谢性放射性标记的人类黑色素瘤细胞系VUP 1中免疫沉淀出一种具有相似电泳迁移率的蛋白质。在来自健康供体的PHA诱导的外周血单个核细胞或EBV转化的外周血单个核细胞上均未检测到HBCA-12单克隆抗体识别的抗原的表达。

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Neoplasma. 1984;31(6):625-9.

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