Laue T M, Yphantis D A, Rhodes D G
Anal Biochem. 1984 Nov 15;143(1):103-12. doi: 10.1016/0003-2697(84)90563-3.
The approaches presented in this series of papers make possible rapid gathering, reduction, and analysis of data from the Rayleigh interference optical system of an analytical ultracentrifuge. Instrumentation described in this paper provides some of the timing and measurement circuits necessary for a microprocessor or minicomputer to determine the rotor frequency, rotor period, and elapsed time of an experiment. It includes simple but effective circuits to generate precise rotor timing pulses that are useful for synchronization of pulsed light sources. Circuits to control photographic operations in the ultracentrifuge are described briefly. All of these circuits are interfaced to a simple microcomputer address/data bus. An adapter between this bus and a Q-bus (for a DEC LSI-11/2 or LSI 11/23 microcomputer) is also described. The circuits presented have been used in this laboratory over a 3-year period. They have proven reliable and form an integral part of the real-time data acquisition systems that have been constructed.
本系列论文中介绍的方法使得从分析型超速离心机的瑞利干涉光学系统中快速收集、处理和分析数据成为可能。本文所描述的仪器提供了一些必要的定时和测量电路,以便微处理器或小型计算机确定转子频率、转子周期以及实验的 elapsed time。它包括简单但有效的电路,用于生成精确的转子定时脉冲,这对于脉冲光源的同步非常有用。简要描述了用于控制超速离心机中摄影操作的电路。所有这些电路都连接到一个简单的微型计算机地址/数据总线上。还描述了该总线与Q总线(用于DEC LSI-11/2或LSI 11/23微型计算机)之间的适配器。本文介绍的电路在本实验室已使用了三年。它们已被证明是可靠的,并构成了已构建的实时数据采集系统的一个组成部分。