从大鼠脑提取物凝胶中纯化肌动蛋白。

Actin purification from a gel of rat brain extracts.

作者信息

Levilliers N, Peron-Renner M, Coffe G, Pudles J

出版信息

Biochimie. 1984 Jul-Aug;66(7-8):531-7. doi: 10.1016/0300-9084(84)90147-0.

Abstract

Actin, 99% pure, has been recovered from rat brain with a high yield (greater than 15 mg/100 g brain). We have shown that: 1. a low ionic strength extract from rat brain tissue is capable of giving rise to a gel; 2. actin is the main gel component and its proportion is one order of magnitude higher than in the original extract; 3. actin can be isolated from this extract by a three-step procedure involving gelation, dissociation of the gel in 0.6 M KCl, followed by one or two depolymerization-polymerization cycles.

摘要

已从大鼠脑中以高产率（大于15毫克/100克脑）回收了纯度为99%的肌动蛋白。我们已经证明：1. 大鼠脑组织的低离子强度提取物能够形成凝胶；2. 肌动蛋白是主要的凝胶成分，其比例比原始提取物高一个数量级；3. 肌动蛋白可以通过三步程序从该提取物中分离出来，该程序包括凝胶化、在0.6M氯化钾中使凝胶解离，然后进行一到两个解聚-聚合循环。