通过亲和色谱法研究脂肪酶-辅脂肪酶相互作用的进一步结果。

Further results on lipase-colipase interactions studied by affinity chromatography.

作者信息

Alessandri J M, Léger C, Mahé N

出版信息

Biochimie. 1984 Nov-Dec;66(11-12):663-72. doi: 10.1016/0300-9084(84)90255-4.

Abstract

Affinity chromatography of lipase on a colipase-coupled gel was studied in the present paper. The elution volume of the associable lipase increased when the loaded amount decreased. A KD value of 1.9 X 10(-6) M at pH 6.2 was thus deduced. A minimum value of 1.5 X 10(-6) M was obtained at pH 5.1-5.3. Mixed micelles associated with coupled colipase, but no modifications of lipase-colipase interactions took place when mixed micelles were added to the elution buffer. DMMA-modified coupled colipase failed to interact with lipase, owing to the specific orientation of the modified cofactor in the gel.

摘要

本文研究了脂肪酶在共脂肪酶偶联凝胶上的亲和色谱。当负载量降低时，可结合脂肪酶的洗脱体积增加。由此推导出在pH 6.2时KD值为1.9×10⁻⁶ M。在pH 5.1 - 5.3时获得的最小值为1.5×10⁻⁶ M。混合胶束与偶联的共脂肪酶相关联，但当将混合胶束添加到洗脱缓冲液中时，脂肪酶 - 共脂肪酶相互作用没有发生改变。由于修饰的辅因子在凝胶中的特定取向，DMMA修饰的偶联共脂肪酶未能与脂肪酶相互作用。