Raison R L, Hildemann W H
Dev Comp Immunol. 1984 Winter;8(1):99-108. doi: 10.1016/0145-305x(84)90014-4.
The occurrence of immunoglobulin (Ig) bearing leucocytes in the blood of the Pacific hagfish, Eptatretus stoutii , was examined using a murine monoclonal antibody (45.3) and a rabbit antiserum specific for hagfish serum Ig. Binding of antibody 45.3 to hagfish leucocytes assessed by radioimmunoassay was inhibited by preincubation of antibody with purified serum Ig thus verifying the presence of cell surface Ig cross reactive with serum Ig. The monoclonal antibody identified approximately 65% of blood leucocytes as Ig+ve while the rabbit antiserum indicated 81% Ig+ve cells. Both antibody preparations failed to react specifically with cells from mouse, horned shark, tunicate or sea star; this indicates the distinctive nature of hagfish Ig. The high percentage of blood cells bearing surface Ig in the hagfish raises the possibility that lymphocyte divergence to separate B and T pathways may not have occurred in this most primitive vertebrate. Alternatively, an Ig-like specificity characteristic of both "T" and "B" lymphocytes may have been detected. In any event, a subset of Ig negative leucocytes is evident in hagfish.
利用鼠单克隆抗体（45.3）和针对盲鳗血清免疫球蛋白（Ig）的兔抗血清，检测了太平洋盲鳗（Eptatretus stoutii）血液中携带Ig的白细胞的出现情况。通过放射免疫测定法评估，抗体45.3与盲鳗白细胞的结合可被抗体与纯化血清Ig的预孵育所抑制，从而证实了存在与血清Ig交叉反应的细胞表面Ig。单克隆抗体鉴定出约65%的血液白细胞为Ig阳性，而兔抗血清显示81%的细胞为Ig阳性。两种抗体制剂均未与来自小鼠、角鲨、被囊动物或海星的细胞发生特异性反应；这表明盲鳗Ig具有独特性质。盲鳗血液中携带表面Ig的血细胞比例很高，这增加了在这种最原始的脊椎动物中可能尚未发生淋巴细胞分化为独立的B细胞和T细胞途径的可能性。或者，可能检测到了“T”和“B”淋巴细胞共有的Ig样特异性特征。无论如何，在盲鳗中明显存在一部分Ig阴性白细胞。
