一种使用生物素新衍生物生物素基-6-氨基喹啉的生物素酶灵敏荧光速率测定法。
A sensitive fluorimetric rate assay for biotinidase using a new derivative of biotin, biotinyl-6-aminoquinoline.
作者信息
Wastell H, Dale G, Bartlett K
出版信息
Anal Biochem. 1984 Jul;140(1):69-73. doi: 10.1016/0003-2697(84)90134-9.
PMID:6548338
Abstract
The previously reported method for the estimation of biotinidase (EC 3.5.1.12) is an endpoint colorimetric assay based on the hydrolysis of biotinyl-4-aminobenzoate, followed by diazotization, and is not suitable for our studies of biotinidase. A fluorimetric rate assay of biotinidase which uses a newly synthesized derivative biotinyl-6-aminoquinoline is described here.
摘要
先前报道的生物素酶（EC 3.5.1.12）估测方法是一种基于生物素基-4-氨基苯甲酸水解、随后重氮化反应的终点比色法，不适用于我们对生物素酶的研究。本文描述了一种使用新合成的衍生物生物素基-6-氨基喹啉的生物素酶荧光速率测定法。
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