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菠菜、向日葵及其他四种还原性戊糖磷酸途径植物叶绿体提取物中核酮糖二磷酸羧化酶动力学特性的比较

Comparison of the kinetic properties of ribulose bisphosphate carboxylase in chloroplast extracts of spinach, sunflower and four other reductive pentose phosphate-pathway species.

作者信息

Delaney M E, Walker D A

出版信息

Biochem J. 1978 May 1;171(2):477-82. doi: 10.1042/bj1710477.

DOI:10.1042/bj1710477
PMID:656056
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183978/
Abstract

Extracts from chloroplasts of spinach, sunflower and four other reductive pentose phosphate (C3)-pathway species were measured spectrophotometrically with or without a modified preactivation procedure. In all six species this modification yielded Km (CO2) values in the range of 7-15 micron and maximum velocities, at 20 degrees C, of 129-431 mumol of CO2 carboxylated/h per mg of chlorophyll. In general, both the carboxylation and electron-transport capacities of sunflower were somewhat greater than that of the other species, and this is discussed in relation to the superior rates of photosynthesis believed to be displayed by the parent tissue.

摘要

对菠菜、向日葵以及其他四种还原性戊糖磷酸(C3)途径植物的叶绿体提取物进行了分光光度测定,测定时采用或不采用改良的预激活程序。在所有这六个物种中,这种改良产生的Km(CO2)值在7-15微米范围内,在20摄氏度时,最大反应速度为每毫克叶绿素每小时羧化129-431微摩尔CO2。总体而言,向日葵的羧化能力和电子传递能力均略高于其他物种,这一点结合母组织被认为具有的较高光合作用速率进行了讨论。

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1
Comparison of the kinetic properties of ribulose bisphosphate carboxylase in chloroplast extracts of spinach, sunflower and four other reductive pentose phosphate-pathway species.菠菜、向日葵及其他四种还原性戊糖磷酸途径植物叶绿体提取物中核酮糖二磷酸羧化酶动力学特性的比较
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引用本文的文献

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Plant Physiol. 1980 Dec;66(6):1110-2. doi: 10.1104/pp.66.6.1110.

本文引用的文献

1
Carbon dioxide assimilation by leaves, isolated chloroplasts, and ribulose bisphosphate carboxylase from spinach.菠菜叶片、分离的叶绿体和核酮糖二磷酸羧化酶对二氧化碳的同化作用。
Plant Physiol. 1975 Jun;55(6):1087-92. doi: 10.1104/pp.55.6.1087.
2
Ribulose Diphosphate Carboxylase from Freshly Ruptured Spinach Chloroplasts Having an in Vivo Km[CO(2)].新鲜破裂菠菜叶绿体中的核酮糖二磷酸羧化酶,其体内 Km[CO(2)]。
Plant Physiol. 1974 Jan;53(1):39-44. doi: 10.1104/pp.53.1.39.
3
The reductive pentose phosphate cycle. III. Enzyme activities in cell-free extracts of photosynthetic organisms.还原戊糖磷酸循环。III. 光合生物无细胞提取物中的酶活性。
Plant Physiol. 1961 Jul;36(4):409-14. doi: 10.1104/pp.36.4.409.
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Statistical estimations in enzyme kinetics.酶动力学中的统计估计
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5
The enzymatic formation of phosphoglyceric acid from ribulose diphosphate and carbon dioxide.由二磷酸核酮糖和二氧化碳酶促形成磷酸甘油酸。
J Biol Chem. 1956 Feb;218(2):795-810.
6
Photosynthesis in Rhodospirillum rubrum. IV. Isolation and characterization of ribulose 1,5-diphosphate carboxylase.深红红螺菌中的光合作用。IV. 1,5-二磷酸核酮糖羧化酶的分离与特性
J Biol Chem. 1969 Jun 25;244(12):3105-9.
7
The active species of "CO2" utilized by ribulose diphosphate carboxylase.二磷酸核酮糖羧化酶所利用的“CO₂”的活性形式。
J Biol Chem. 1969 Feb 10;244(3):1081-3.
8
An ALGOL program for the computer-assisted calculation of enzyme kinetic data.一个用于计算机辅助计算酶动力学数据的ALGOL程序。
Int J Biomed Comput. 1971 Jan;2(1):71-7. doi: 10.1016/0020-7101(71)90020-1.
9
Effects of CO2, O2 and temperature on a high-affinity form of ribulose diphosphate carboxylase-oxygenase from spinach.二氧化碳、氧气和温度对菠菜中一种高亲和力形式的二磷酸核酮糖羧化酶加氧酶的影响。
Biochem Biophys Res Commun. 1974 Sep 9;60(1):204-10. doi: 10.1016/0006-291x(74)90192-2.
10
An improved spectrophotometric assay for ribulosebisphosphate carboxylase.一种改进的核酮糖二磷酸羧化酶分光光度测定法。
Biochim Biophys Acta. 1974 Jul 17;358(1):226-9. doi: 10.1016/0005-2744(74)90274-5.