人抗凝血酶III cDNA克隆的分离与序列特征分析
Isolation and sequence characterization of a cDNA clone of human antithrombin III.
作者信息
Chandra T, Stackhouse R, Kidd V J, Woo S L
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1845-8. doi: 10.1073/pnas.80.7.1845.
Abstract
A human liver cDNA library was constructed by using poly(A)-containing RNA isolated from a human liver biopsy specimen. This library is comprised of 40,000 independent transformants with an average inserted DNA length of 1,200 base pairs. By using the previously cloned baboon antithrombin III cDNA as a specific hybridization probe, greater than 30 human antithrombin III cDNA clones were identified from this library. The clone with the longest DNA insert was selected for sequence analysis. This antithrombin III cDNA clone contains 1,479 base pairs of inserted human DNA and was designated phATIII 113. It contains DNA sequences that code for a signal peptide and the entire mature antithrombin III protein which is comprised of 432 amino acid residues.
摘要
通过使用从人肝活检标本中分离出的含多聚腺苷酸（poly(A)）的RNA构建了人肝脏cDNA文库。该文库由40,000个独立的转化体组成，平均插入DNA长度为1200个碱基对。通过使用先前克隆的狒狒抗凝血酶III cDNA作为特异性杂交探针，从该文库中鉴定出了30多个人类抗凝血酶III cDNA克隆。选择插入DNA最长的克隆进行序列分析。这个抗凝血酶III cDNA克隆包含1479个碱基对的插入人类DNA，被命名为phATIII 113。它包含编码信号肽和由432个氨基酸残基组成的整个成熟抗凝血酶III蛋白的DNA序列。
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