大鼠脑中的3α-羟基类固醇氧化还原酶

3 alpha-Hydroxysteroid oxidoreductase in rat brain.

作者信息

Krieger N R, Scott R G

出版信息

J Neurochem. 1984 Mar;42(3):887-90. doi: 10.1111/j.1471-4159.1984.tb02767.x.

DOI:10.1111/j.1471-4159.1984.tb02767.x

Abstract

We describe a simple procedure for the microassay of 3 alpha-hydroxysteroid oxidoreductase in homogenates of rat brain. This enzyme converts dihydrotestosterone to 3 alpha-androstandiol. We have mapped the distribution of the enzymatic activity in 14 regions of the rat brain. The highest activities were observed in homogenates of olfactory bulb (51/nmol/mg protein/h) and olfactory tubercle (29 nmol/mg protein/h). Substantially lower values were seen in the other brain regions, including thalamus, caudate nucleus, frontal cortex, hippocampus, hypothalamus, and preoptic area (6-20 nmol/mg protein/h).

摘要

我们描述了一种用于大鼠脑匀浆中3α-羟基类固醇氧化还原酶微量测定的简单方法。该酶可将二氢睾酮转化为3α-雄烷二醇。我们已绘制出该酶活性在大鼠脑14个区域中的分布图。在嗅球匀浆（51/纳摩尔/毫克蛋白/小时）和嗅结节匀浆（29纳摩尔/毫克蛋白/小时）中观察到最高活性。在包括丘脑、尾状核、额叶皮质、海马体、下丘脑和视前区在内的其他脑区中，活性值则显著较低（6 - 20纳摩尔/毫克蛋白/小时）。