Schultz D R, Arnold P I
Acta Pathol Microbiol Immunol Scand Suppl. 1984;284:59-66.
A method is described for isolating both functionally and highly pure C2 from normal human serum or plasma. Functionally pure C2 was obtained by a two-step method of ammonium sulfate (AS) fractionation of plasma or serum and chromatography on AH-Sepharose containing a bound lectin of Euonymus europeus. The functionally pure C2 can be isolated in 2 days, and is used routinely as a reagent for functional hemolytic titrations of C3 and C4 in the Clinical Immunology Laboratory. Highly pure C2 was isolated by the two-step fractionation with AS followed by chromatography on CM-cellulose, aged CNBr-activated Sepharose 4B, and AH-Sepharose-lectin. The major difficulty for isolating highly pure C2 was its separation from Factor B of the alternative complement pathway. The yield of C2 varied from 30 to 40 per cent. Following electrophoresis and staining on polyacrylamide gels, the single band was hemolytically active C2.
本文描述了一种从正常人血清或血浆中分离功能上纯净且高度纯化的C2的方法。通过两步法对血浆或血清进行硫酸铵（AS）分级分离，并在含有欧洲卫矛凝集素的AH-琼脂糖上进行色谱分离，获得功能上纯净的C2。功能上纯净的C2可在2天内分离出来，并在临床免疫实验室中常规用作C3和C4功能溶血滴定的试剂。通过AS两步分级分离，随后在CM-纤维素、老化的溴化氰活化琼脂糖4B和AH-琼脂糖-凝集素上进行色谱分离，获得高度纯化的C2。分离高度纯化的C2的主要困难在于将其与替代补体途径的B因子分离。C2的产率在30%至40%之间。在聚丙烯酰胺凝胶上进行电泳和染色后，单一条带为具有溶血活性的C2。
