[75硒]硒代蛋氨酸在免疫球蛋白生物合成研究中的应用。
Use of [75Se]selenomethionine in immunoglobulin biosynthetic studies.
作者信息
Gutman G A, Warner N L, Harris A W, Bowles A
出版信息
J Immunol Methods. 1978;21(1-2):101-9. doi: 10.1016/0022-1759(78)90227-2.
The gamma-emitting amino acid analog, [75Se]selenomethionine, has been used as a biosynthetic label for immunoglobulins secreted by plasmacytomas in tissue culture. The secreted products are structurally intact with respect to their antibody combining sites and their class and allotype antigenic specificities. A component of [75Se]selenomethionine preparations was found to bind to fetal calf serum proteins, in a manner releasable by mercaptoethanol, but not by sodium dodecyl sulfate (SDS) and urea. Methods for circumventing the problems caused by this binding are described.
发射γ射线的氨基酸类似物[75Se]硒代蛋氨酸已被用作组织培养中浆细胞瘤分泌的免疫球蛋白的生物合成标记物。所分泌的产物在其抗体结合位点、类别和同种异型抗原特异性方面结构完整。发现[75Se]硒代蛋氨酸制剂的一种成分以可被巯基乙醇释放但不能被十二烷基硫酸钠（SDS）和尿素释放的方式与胎牛血清蛋白结合。本文描述了规避这种结合所引起问题的方法。
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