Ida T, Sato M, Yamaoka Y, Takeda H, Kamiyama Y
J Lab Clin Med. 1976 Jun;87(6):925-33.
The effect of insulin was investigated in the isolated guinea pig liver perfused with Krebs-Ringer bicarbonate buffer containing red blood cells and albumin. In the mitochondria isolated from livers perfused with 10 units of insulin per hour, the phosphorylative activity with glutamate as a substrate increased to about 160 per cent of control 60 minutes after the beginning of perfusion (p less than 0.01). Such an enhanced phosphorylative activity was accompanied by increases in the respiratory control ratio, state 3 respiration, and P/O ratio. On the other hand, in the liver perfused with insulin, the levels of the energy charge and adenine nucleotide quotient increased to a significant degree as compared to the liver without insulin (p less than 0.01 and p less than 0.05, respectively). It is suggested that insulin plays an important role as a portal factor in regulating mitochondrial oxidative phosphorylation and the levels of the phosphorylated adenine nucleotides.
在含有红细胞和白蛋白的 Krebs-Ringer 碳酸氢盐缓冲液灌注的离体豚鼠肝脏中研究了胰岛素的作用。在每小时用 10 单位胰岛素灌注的肝脏分离出的线粒体中，以谷氨酸为底物的磷酸化活性在灌注开始 60 分钟后增加到对照的约 160%（p<0.01）。这种增强的磷酸化活性伴随着呼吸控制率、状态 3 呼吸和 P/O 比值的增加。另一方面，与未灌注胰岛素的肝脏相比，灌注胰岛素的肝脏中能量电荷和腺嘌呤核苷酸商水平显著升高（分别为 p<0.01 和 p<0.05）。提示胰岛素作为门控因子在调节线粒体氧化磷酸化和磷酸化腺嘌呤核苷酸水平方面起重要作用。
