Chen S S, Tung J S, Gillis S, Good R A, Hadden J W
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5980-4. doi: 10.1073/pnas.80.19.5980.
Peanut agglutinin-positive thymocytes, peanut agglutinin-negative thymocytes, cortisone-resistant thymocytes, and unfractionated thymocytes were prepared from congeneic C57BL/6 Tla mice. By using surface iodination and immunoprecipitation of solubilized antigen with specific antisera (e.g., anti-H-2D, anti-TL, anti-Qa2/3, and anti-gp70), the released specific antigens were electrophoresed on polyacrylamide gels, and their radioactivity was measured. The relative percentages of surface antigens H-2D, TL, Qa2/3, and gp70 were 3.2%, 47.5%, 2.5%, and 46.8%, respectively, for peanut agglutinin-positive thymocytes; 31.8%, 4.4%, 32.7%, and 31.1%, respectively, for cortisone-resistant thymocytes; 13.2%, 28.7%, 12.3%, and 45.8%, respectively, for peanut agglutinin-negative thymocytes; and 7.7%, 27.1%, 4.3%, and 60.9%, respectively, for unfractionated thymocytes. After incubation with thymosin fraction V or T-cell growth factor (interleukin II) for 20 hr, the changes in surface antigens of peanut agglutinin-positive thymocytes closely correlated with their normal maturation (i.e., H-2D increases and TL decreases). Thymic factors (e.g., thymosin alpha 1, thymopoietin pentapeptide, facteur thymic serique) had only small or no effects on surface antigens of peanut agglutinin-positive thymocytes. The results suggest that peptides yet to be identified in thymosin fraction V may play an important role in intrathymic evolution and that T-cell growth factor is possibly a peripheral signal derived from activated T cells that modulates T-cell receptors and may be a critical regulator of intrathymic cellular development.
从同基因C57BL/6 Tla小鼠制备花生凝集素阳性胸腺细胞、花生凝集素阴性胸腺细胞、抗可的松胸腺细胞和未分级胸腺细胞。通过表面碘化以及用特异性抗血清（如抗H-2D、抗TL、抗Qa2/3和抗gp70）对溶解抗原进行免疫沉淀，将释放的特异性抗原在聚丙烯酰胺凝胶上进行电泳，并测量其放射性。对于花生凝集素阳性胸腺细胞，表面抗原H-2D、TL、Qa2/3和gp70的相对百分比分别为3.2%、47.5%、2.5%和46.8%；对于抗可的松胸腺细胞，分别为31.8%、4.4%、32.7%和31.1%；对于花生凝集素阴性胸腺细胞，分别为13.2%、28.7%、12.3%和45.8%；对于未分级胸腺细胞，分别为7.7%、27.1%、4.3%和60.9%。用胸腺素组分V或T细胞生长因子（白细胞介素II）孵育20小时后，花生凝集素阳性胸腺细胞表面抗原的变化与其正常成熟密切相关（即H-2D增加而TL减少）。胸腺因子（如胸腺素α1、胸腺生成素五肽、胸腺血清因子）对花生凝集素阳性胸腺细胞的表面抗原只有很小的影响或没有影响。结果表明，胸腺素组分V中尚未鉴定的肽可能在胸腺内进化中起重要作用，并且T细胞生长因子可能是来自活化T细胞的外周信号，可调节T细胞受体，可能是胸腺内细胞发育的关键调节因子。
