Gallati H
J Clin Chem Clin Biochem. 1976 Jan;14(1):15-22.
The oxidoreductase inhibitor was prepared from NAD in alkaline solution, and purified chromatographically. Investigations are reported on the extinction and fluorescence spectra, stability of the inhibitor, and the dependence of inhibition on the concentration of enzyme and coenzyme. Kinetic studies show that the inhibition is non-competitive.
氧化还原酶抑制剂是在碱性溶液中由烟酰胺腺嘌呤二核苷酸(NAD)制备而成,并通过色谱法进行纯化。本文报道了关于该抑制剂的消光光谱和荧光光谱、稳定性以及抑制作用对酶和辅酶浓度的依赖性的研究。动力学研究表明,这种抑制作用是非竞争性的。