通过氧化还原电位滴定法和光谱法解析羟胺氧化还原酶的血红素
Resolution of the hemes of hydroxylamine oxidoreductase by redox potentiometry and optical spectroscopy.
作者信息
Prince R C, Larroque C, Hooper A B
出版信息
FEBS Lett. 1983 Oct 31;163(1):25-7. doi: 10.1016/0014-5793(83)81154-5.
PMID:6628687
Abstract
Optical spectroscopy combined with redox potentiometry has resolved the hemes of hydroxylamine oxidoreductase into 6 thermodynamically distinct classes. There are apparently 4 classes of heme c553, with Em7-values of 295 mV, 10 mV, -190 mV and -390 mV, present in a stoichiometry of 1:1:2:1; two equivalents of heme c559, Em7 O mV, and one of heme P-460, an unusual chromophore, with Em7 -260 mV.
摘要
光学光谱法与氧化还原电位测定法相结合，已将羟胺氧化还原酶的血红素解析为6个热力学上不同的类别。显然有4类血红素c553，其Em7值分别为295 mV、10 mV、-190 mV和-390 mV，化学计量比为1:1:2:1；有两当量的血红素c559，Em7为0 mV，还有一当量的血红素P-460，一种不寻常的发色团，Em7为-260 mV。
Zotero 插件