Harada S, Agarwal D P, Goedde H W, Takagi S
Pharmacol Biochem Behav. 1983;18 Suppl 1:139-40. doi: 10.1016/0091-3057(83)90161-2.
Aldehyde dehydrogenase (ALDH) isozyme I deficiency in hair root samples from 105 healthy individuals and 72 alcoholics was determined using isoelectric focusing. From these individuals, 12 male alcoholics (2 with ALDH isozyme I deficiency and 10 normal) and 45 healthy controls (18 with ALDH isozyme I deficiency and 27 normal) were investigated for their blood ethanol and acetaldehyde levels by gas chromatography after an acute dose of alcohol (0.5 g ethanol/kg body wt.). Peak blood ethanol values of about 10 mmol/l were attained after 1 hour both in alcoholics and normal controls irrespective of their ALDH type. There was no significant difference in the blood ethanol level during the 5 hr post-drinking period in both the groups. Peak blood acetaldehyde concentration was significantly higher in healthy controls and alcoholics deficient in ALDH isozyme I after alcohol drinking (about 30 micro-mmol/l) than in individuals with normal ALDH isozyme I (3 micro-mmol/l). However, no significant difference in blood acetaldehyde was observed between alcoholics and controls.
采用等电聚焦法测定了105名健康个体和72名酗酒者发根样本中的乙醛脱氢酶（ALDH）同工酶I缺乏情况。从这些个体中选取了12名男性酗酒者（2名ALDH同工酶I缺乏，10名正常）和45名健康对照者（18名ALDH同工酶I缺乏，27名正常），在急性摄入酒精（0.5 g乙醇/千克体重）后，通过气相色谱法检测他们的血液乙醇和乙醛水平。无论ALDH类型如何，酗酒者和正常对照者在1小时后血液乙醇峰值均达到约10 mmol/l。两组在饮酒后5小时内的血液乙醇水平无显著差异。饮酒后，健康对照者和ALDH同工酶I缺乏的酗酒者的血液乙醛峰值浓度（约30微摩尔/升）显著高于ALDH同工酶I正常的个体（3微摩尔/升）。然而，酗酒者和对照者之间的血液乙醛水平未观察到显著差异。
