Leung A F
J Muscle Res Cell Motil. 1983 Oct;4(5):557-68. doi: 10.1007/BF00712115.
The intensity of the diffraction line, the transverse intensity, of a single skeletal muscle fibre illuminated at normal incidence with a laser beam decayed monotonically to zero at both ends of the diffraction line. This characteristic decay was observed for most illuminated spots of the fibre. However, for a few spots the transverse intensity showed distinctive oscillations. The intensity distribution of the diffraction line is explained by a diffraction model in which the muscle fibre acts as a bundle of randomly packed myofibrils of different diameters. The equatorial intensity was calculated as a function of myofibrillar diameter dispersion. The comparison between the theoretical curves and measurements yields an estimation for the myofibrillar diameter dispersion. The calculations predict that the transverse intensity would exhibit oscillations when the diameter dispersion is less than 22%. Finally, the diameter dispersions estimated by light diffractometry and electron microscopy are compared.
用激光束垂直照射单个骨骼肌纤维时，衍射线的强度，即横向强度，在衍射线两端单调衰减至零。在纤维的大多数照射点都观察到了这种特征性衰减。然而，对于少数几个点，横向强度显示出明显的振荡。衍射线的强度分布由一个衍射模型解释，在该模型中，肌肉纤维充当不同直径的随机排列的肌原纤维束。计算了赤道强度作为肌原纤维直径分散度的函数。理论曲线与测量值之间的比较得出了肌原纤维直径分散度的估计值。计算预测，当直径分散度小于22%时，横向强度将呈现振荡。最后，比较了通过光衍射法和电子显微镜估计的直径分散度。
