Obrist R, Sandberg A L
Cell Immunol. 1983 Oct 1;81(1):169-74. doi: 10.1016/0008-8749(83)90222-8.
The numbers of macrophages in peritoneal guinea pig hepatomas were significantly (P less than 0.005) elevated by the intraperitoneal injection of a covalent conjugate of the chemotactic peptide, formylmethionylleucylphenylalanine (fMLP), and IgG reactive with surface antigens on the hepatoma cells. These conjugates, which were previously shown to be chemotactic for guinea pig peritoneal exudate macrophages in vitro, increased the numbers of macrophages in the tumors approximately twofold when injected either in a single dose or in five doses. Although five injections of unconjugated fMLP were nearly as effective as the IgG-fMLP conjugates, free fMLP did not enhance the numbers of macrophages in tumors when injected as a single dose. Unconjugated IgG had no effect. The mean tumor weights were decreased in those groups of guinea pigs which received IgG-fMLP but statistical significance was not achieved due to tumor weight variability in all groups.
通过腹腔注射趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）与可与肝癌细胞表面抗原反应的IgG的共价缀合物，豚鼠腹腔肝癌中的巨噬细胞数量显著增加（P小于0.005）。这些缀合物先前已证明在体外对豚鼠腹腔渗出巨噬细胞具有趋化作用，当以单剂量或五剂量注射时，可使肿瘤中的巨噬细胞数量增加约两倍。尽管五次注射未缀合的fMLP几乎与IgG-fMLP缀合物一样有效，但单剂量注射游离fMLP时并不能增加肿瘤中的巨噬细胞数量。未缀合的IgG没有效果。接受IgG-fMLP的豚鼠组的平均肿瘤重量有所降低，但由于所有组的肿瘤重量存在差异，未达到统计学显著性。
