Mladenovic J, Adamson J W
Br J Haematol. 1984 Feb;56(2):323-32. doi: 10.1111/j.1365-2141.1984.tb03959.x.
The effect of hormonal interactions on human erythroid colony growth has been studied using the beta adrenergic agonist, isoproterenol. The growth of colonies derived from both erythroid burst-forming cells (BFU-E) and erythroid colony-forming cells (CFU-E) was enhanced in the presence of isoproterenol. While isoproterenol was effective at all concentrations of erythropoietin (Ep) in cultures of marrow cells, an increase in BFU-E-derived colonies in peripheral blood cultures could be detected only at suboptimal levels of Ep concentrations. The isoproterenol effect was blocked by an agent with beta 2 receptor specificity (butoxamine), and L-isomeric configuration (L-propranolol). The stimulatory effect appeared to be mediated by a receptor different from that for Ep or for phytohaemagglutinin-stimulated leucocyte conditioned medium. Circulating BFU-E from eight patients with polycythaemia vera were also studied. Those colonies which grew in the absence of added Ep increased with isoproterenol; in cultures from normal subjects and in patients with secondary erythrocytosis, agonist stimulation was seen only in the presence of Ep. These studies provide evidence that the growth of both normal and neoplastic erythroid progenitors may be modulated by hormonal interactions.
已使用β肾上腺素能激动剂异丙肾上腺素研究了激素相互作用对人红系集落生长的影响。在异丙肾上腺素存在的情况下，源自红系爆式集落形成细胞（BFU-E）和红系集落形成细胞（CFU-E）的集落生长均得到增强。虽然异丙肾上腺素在骨髓细胞培养物中的所有促红细胞生成素（Ep）浓度下均有效，但仅在Ep浓度处于次优水平时，才能检测到外周血培养物中源自BFU-E的集落增加。异丙肾上腺素的作用可被具有β2受体特异性的药物（布托沙明）和L-异构体构型（L-普萘洛尔）阻断。刺激作用似乎是由一种不同于Ep或植物血凝素刺激的白细胞条件培养基的受体介导的。还研究了8例真性红细胞增多症患者的循环BFU-E。那些在未添加Ep的情况下生长的集落随着异丙肾上腺素而增加；在正常受试者和继发性红细胞增多症患者的培养物中，仅在有Ep存在时才可见激动剂刺激。这些研究提供了证据，表明正常和肿瘤性红系祖细胞的生长可能受到激素相互作用的调节。
