11S大鼠精囊mRNA指导主要分泌蛋白IV的两种前体在体外合成。

The 11S rat seminal vesicle mRNA directs the in vitro synthesis of two precursors of the major secretory protein IV.

作者信息

Metafora S, Guardiola J, Paonessa G, Abrescia P

出版信息

Nucleic Acids Res. 1984 Feb 24;12(4):1901-10. doi: 10.1093/nar/12.4.1901.

DOI:10.1093/nar/12.4.1901

PMID:6701092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318629/

Abstract

The 11s mRNA extracted from the rat seminal vesicles directs the synthesis of two different precursors of the major secretory protein RSV-IV. These two precursors are not interconvertible and seemingly originate from different translational events. Sucrose gradients, polyacrylamide gel electrophoresis and positive hybridization translation experiments do not allow the separation of the two putatively different mRNAs. It is concluded that the two RSV-IV precursors either derive from two extremely similar, but physically not separable mRNA species, or from two different modes of translation of the same mRNA molecule.

摘要

从大鼠精囊中提取的11s mRNA指导主要分泌蛋白RSV-IV的两种不同前体的合成。这两种前体不能相互转化，似乎起源于不同的翻译事件。蔗糖梯度、聚丙烯酰胺凝胶电泳和阳性杂交翻译实验无法分离这两种推测不同的mRNA。结论是，这两种RSV-IV前体要么来自两种极其相似但在物理上无法分离的mRNA种类，要么来自同一mRNA分子的两种不同翻译模式。