对三个种马场的马传染性子宫炎进行血清学和细菌学调查。

Serological and bacteriological survey of three horse studs for contagious equine metritis.

作者信息

Dolan M, Cargill C, Martin F, Davenport P, Franks D, Lightfoot J

出版信息

Aust Vet J. 1984 Jan;61(1):17-9. doi: 10.1111/j.1751-0813.1984.tb07123.x.

DOI:10.1111/j.1751-0813.1984.tb07123.x

Abstract

A bacteriological and serological survey for evidence of contagious equine metritis (CEM) was made during the 1980 breeding season on 3 horse studs in South Australia with a history of previous infection. Swabs from the clitoral sinus and the cervix were cultured for Haemophilus equigenitalis and serum was screened for antibody using the complement fixation test (CFT) and the enzyme-linked immunosorbent assay (ELISA). The specificity of both tests was greater than 0.99 but the ELISA was more sensitive in detecting antibody in infected mares. On the evidence presented it was concluded that H. equigenitalis is no longer present in the horse studs investigated.

摘要

1980年繁殖季节期间，对南澳大利亚3个曾有过感染史的种马场进行了一项细菌学和血清学调查，以寻找马传染性子宫炎（CEM）的证据。采集阴蒂窦和子宫颈拭子培养马生殖支原体，并用补体结合试验（CFT）和酶联免疫吸附测定（ELISA）检测血清中的抗体。两种检测方法的特异性均大于0.99，但ELISA在检测感染母马的抗体方面更敏感。根据所提供的证据得出结论，在所调查的种马场中已不存在马生殖支原体。