Martin D R, Dick K J
J Med Microbiol. 1984 Apr;17(2):189-99. doi: 10.1099/00222615-17-2-189.
Sequential serum samples obtained from 50 rheumatic fever subjects and from control individuals matched for time, age and geographical location were tested for antibodies against the M-associated protein antigens, MAP I and MAP II. Antibody titres were determined by the complement fixation test with a partially-purified extract of Streptococcus pyogenes serotype M30 as the MAP I antigen and an acid extract of serotype M48 as the MAP II antigen. Titres of MAP I antibody exceeded those of MAP II antibody in all but six rheumatic fever subjects. Anti-MAP I titres in excess of 40 were significantly more common in rheumatic fever subjects than in matched controls (p less than 0.001) or matched subjects with a diagnosis of acute post-streptococcal glomerulonephritis (p less than 0.01). Peak MAP I titres were present at the time of admission to hospital in the sera of 40 of the 50 rheumatic fever subjects. In the remainder peak titres occurred within 10 days. Antibody titres were maintained for a mean of 10.3 weeks before declining. Changes in MAP antibody titres were independent of changes in antistreptolysin O and anti-DNAase B titres. Normal children aged between 6 and 15 had higher MAP antibody titres than 2-5-year-old children. Rheumatic fever subjects had significantly higher mean titres of MAP I antibody than matched controls in each age group.
从50名风湿热患者以及在时间、年龄和地理位置上与之匹配的对照个体中获取序贯血清样本，检测其针对M相关蛋白抗原MAP I和MAP II的抗体。抗体滴度通过补体结合试验测定，以化脓性链球菌M30血清型的部分纯化提取物作为MAP I抗原，以M48血清型的酸提取物作为MAP II抗原。除6名风湿热患者外，所有患者的MAP I抗体滴度均超过MAP II抗体滴度。风湿热患者中抗MAP I滴度超过40的情况显著多于匹配的对照组（p<0.001）或诊断为急性链球菌感染后肾小球肾炎的匹配受试者（p<0.01）。50名风湿热患者中有40名患者入院时血清中MAP I滴度达到峰值。其余患者的峰值滴度在10天内出现。抗体滴度在下降前平均维持10.3周。MAP抗体滴度的变化与抗链球菌溶血素O和抗DNA酶B滴度的变化无关。6至15岁的正常儿童的MAP抗体滴度高于2至5岁的儿童。在每个年龄组中，风湿热患者的MAP I抗体平均滴度显著高于匹配的对照组。
