RNA聚合酶II的显微注射可纠正tsAF8细胞的温度敏感缺陷。

Microinjection of RNA polymerase II corrects the temperature-sensitive defect of tsAF8 cells.

作者信息

Waechter D E, Avignolo C, Freund E, Riggenbach C M, Mercer W E, McGuire P M, Baserga R

出版信息

Mol Cell Biochem. 1984;60(1):77-82. doi: 10.1007/BF00226301.

Abstract

tsAF8 cells are a temperature-sensitive (ts) mutant of BHK cells that arrest in the G1 phase of the cell cycle at the non-permissive temperature of 40.6 degrees C. Previous reports had suggested that the temperature-sensitivity of these cells was based on a defect in either the synthesis, assembly or turnover of RNA polymerase II. We now show that the direct microinjection of purified RNA polymerase II into nuclei of tsAF8 cells corrects the ts defect and allows these cells to enter the S phase of the cell cycle.

摘要

tsAF8细胞是BHK细胞的温度敏感（ts）突变体，在40.6摄氏度的非允许温度下会停滞在细胞周期的G1期。先前的报告表明，这些细胞的温度敏感性是基于RNA聚合酶II的合成、组装或周转缺陷。我们现在表明，将纯化的RNA聚合酶II直接显微注射到tsAF8细胞的细胞核中可纠正ts缺陷，并使这些细胞进入细胞周期的S期。