神经元可诱导仅含有肌肉发育不全细胞核的肌管发生收缩。

Neurons induce contractions in myotubes containing only muscular dysgenic nuclei.

作者信息

Powell J A, Peterson A, Paul C V

出版信息

Muscle Nerve. 1984 Mar-Apr;7(3):204-10. doi: 10.1002/mus.880070304.

Abstract

Muscular dysgenic (mdg/mdg) myotubes cultured alone do not contract. Glucosephosphate isomerase (GPI-1) isozymes were analyzed to determine the final genotype of cultured dysgenic (mdg/mdg, gpi-1a/a) myotubes to which normal embryonic spinal cord and limb cells (CBA/J +/+, gpi-1b/b) had been added. Although both myoblast and spinal cord cell additions caused induction of spontaneous contractions, only myoblasts fused with the myotubes to create functional heterokaryons. Spinal cord cells did not fuse with dysgenic myotubes, but formed functional synapses.

摘要

单独培养的肌肉发育不全（mdg/mdg）肌管不会收缩。分析葡萄糖磷酸异构酶（GPI-1）同工酶，以确定已添加正常胚胎脊髓和肢体细胞（CBA/J +/+, gpi-1b/b）的培养发育不全（mdg/mdg, gpi-1a/a）肌管的最终基因型。虽然添加成肌细胞和脊髓细胞都会引起自发收缩的诱导，但只有成肌细胞与肌管融合形成功能性异核体。脊髓细胞不会与发育不全的肌管融合，但会形成功能性突触。