Vassetzky S G, Sekirina G G, Veisman B L, Skoblina M N, Bilinkis A A
Cell Differ. 1984 Apr;14(1):47-52. doi: 10.1016/0045-6039(84)90007-1.
The oocytes of the starfish Aphelasterias japonica were divided into anuclear (cytoplasts) and nuclear (germinal vesicles) fragments by centrifugation in a Ficoll gradient with cytochalasin B. Cell hybrids between the oocytes and the cytoplasts were then prepared with the aid of polyethylene glycol treatment. These cell hybrids were capable of responding to 1-methyladenine by maturation.
通过在含有细胞松弛素B的Ficoll梯度中离心，将日本单肢海星的卵母细胞分为无核（细胞质体）和有核（生发泡）片段。然后借助聚乙二醇处理制备卵母细胞与细胞质体之间的细胞杂种。这些细胞杂种能够通过成熟对1-甲基腺嘌呤作出反应。
Zotero 插件