Biochemical difference between ventral and dorsolateral prostates: effects of castration and administration of the antiandrogen 16 beta-ethyl-17 beta-hydroxy-4-oestren-3-one on SDS-electrophoretic patterns of subcellular proteins from rat prostates.

Protein species in the cytosol, postnuclear particulate, and nuclear fractions from the ventral and dorsolateral prostates of rats castrated or injected with antiandrogen, 16 beta-ethyl-17 beta-hydroxy-4- oestren -3-one ( EHOO ), were analyzed by SDS-polyacrylamide gel electrophoresis. The effects of EHOO injection on the contents of the most abundant species in all three fractions of ventral prostate were similar to the effects of castration, but the effects of EHOO and castration were only similar on the nuclear fraction in the dorsolateral prostate. The most sensitive species to castration in the three subcellular fractions of dorsolateral prostate were the same as those to EHOO injections; this was not the case for the cytosol and nuclear fractions in the ventral prostate.