Björck S, Ahlman H, Dahlström A
Gut. 1984 Jun;25(6):603-10. doi: 10.1136/gut.25.6.603.
The influence on the concentrating ability of the gall bladder after extrinsic denervation was studied in anaesthetised cats, previously subjected to truncal vagotomy and/or coeliacectomy , and compared with sham operated controls. Net water absorption was studied by perfusion techniques. Acute experiments were performed under basal conditions and alpha-adrenoceptor stimulation (intra-arterial infusion of noradrenaline). Gall bladder biopsies were studied by fluorescence microscopy and cytofluorimetry to visualise and quantify catecholamines. Three weeks after coeliacectomy basal absorption had decreased significantly. In the short term vagotomy group no changes were shown. In the long term vagotomy group, however, there was a four-fold increase in absorptive capacity, which decreased to control levels after alpha-adrenoceptor blockade (phentolamine). Long term vagotomy with subsequent coeliacectomy caused no significant changes. Infusion of noradrenaline increased net water absorption by 60 +/- 11% in all experimental groups except in long term vagotomised animals, where the high basal absorption was not further augmented. One hour after noradrenaline infusion controls returned to basal absorption rate, while denervated cats remained at stimulated levels. In long term vagotomised gall bladders there were morphological signs of adrenergic proliferation (increased total number of nerve terminals, sprouting and raised levels of intraneuronal noradrenaline). These results suggest that the adrenergic nervous system is important for full absorptive capacity of the gall bladder. The increased absorption after long term vagotomy, abolished after alpha-adrenoceptor blockade, might well be explained by the parallel adrenergic proliferation. This hypothesis was further corroborated in animals with long term vagotomy, where subsequent surgical adrenergic denervation restored basal absorption to control levels.
在麻醉猫身上研究了去神经支配对外侧胆囊浓缩能力的影响，这些猫之前已接受迷走神经干切断术和/或腹腔神经节切除术，并与假手术对照组进行比较。通过灌注技术研究净水吸收情况。在基础条件和α-肾上腺素能受体刺激（动脉内注射去甲肾上腺素）下进行急性实验。通过荧光显微镜和细胞荧光测定法研究胆囊活检组织，以可视化和量化儿茶酚胺。腹腔神经节切除术后三周，基础吸收显著下降。短期迷走神经切断组未显示变化。然而，长期迷走神经切断组的吸收能力增加了四倍，在α-肾上腺素能受体阻断（酚妥拉明）后降至对照水平。长期迷走神经切断术随后进行腹腔神经节切除术未引起显著变化。除长期迷走神经切断的动物外，所有实验组中去甲肾上腺素的输注使净水吸收增加了60±11%，在长期迷走神经切断的动物中，高基础吸收未进一步增加。去甲肾上腺素输注一小时后，对照组恢复到基础吸收率，而去神经支配的猫则保持在刺激水平。在长期迷走神经切断的胆囊中，有肾上腺素能增殖的形态学迹象（神经末梢总数增加、发芽和神经元内去甲肾上腺素水平升高）。这些结果表明，肾上腺素能神经系统对胆囊的充分吸收能力很重要。长期迷走神经切断后吸收增加，在α-肾上腺素能受体阻断后消失，很可能由平行的肾上腺素能增殖来解释。这一假设在长期迷走神经切断的动物中得到进一步证实，在这些动物中，随后的手术性肾上腺素能去神经支配使基础吸收恢复到对照水平。
