Lanyi J K
Biochem Biophys Res Commun. 1984 Jul 18;122(1):91-6. doi: 10.1016/0006-291x(84)90443-1.
Two alternative hypotheses have been presented as to the nature of the principal halorhodopsin photointermediate: a) it is a form whose its absorption band is shifted from the 575 nm position to 500 or 520 nm, and b) it is a form whose absorption band is shifted to only about 565 nm, but with an altered band shape so it exhibits a fortuitous difference peak near 500 nm. Such a shift with a maximum near 500 nm is also obtained in the dark when chloride is removed from the sample, suggesting the hypothesis that the spectral changes reflect the transient detachment of chloride from a binding site (Ogurusu et al, J. Biochem. Tokyo 95, 1073-1082, 1984). Comparison of the quantum yields of flash-induced absorption changes in halorhodopsin and bacteriorhodopsin strongly suggests, however, that hypothesis b) is untenable.
关于主要嗜盐视紫红质光中间体的性质，已经提出了两种替代假说：a）它是一种吸收带从575nm位置移至500或520nm的形式，b）它是一种吸收带仅移至约565nm，但具有改变的带形，因此在500nm附近呈现偶然的差异峰的形式。当从样品中除去氯化物时，在黑暗中也会获得在500nm附近具有最大值的这种位移，这表明光谱变化反映了氯化物从结合位点的瞬时脱离的假说（Ogurusu等人，《东京生物化学杂志》95，1073 - 1082，1984）。然而，对嗜盐视紫红质和细菌视紫红质中闪光诱导的吸收变化的量子产率的比较强烈表明，假说b）是站不住脚的。
