Tanaka T, Yamagishi H, Pellis N R, Kahan B D
Cancer Immunol Immunother. 1982;13(1):48-52. doi: 10.1007/BF00200200.
Rabbits immunized with the immunoprotective TSTA fraction partially purified by preparative isoelectric focusing of 3 M KCl extracts from a chemically induced murine sarcoma, MCA-F, produced specific xenoantisera as assessed by an indirect membrane immunofluorescence assay. Only the immunizing tumor, MCA-F, and not the antigenically distinct MCA-D or MCA-T target cells were stained by the xenoantiserum. Absorption of anti-MCA-F antiserum with the antigenically distinct MCA-D or MCA-T cells did not reduce its capacity to bind to MCA-F cells. The immunofluorescence reaction was competitively inhibited by MCA-F fractions that induced specific immunoprotection: crude 3 M KCl extract, isoelectrically focused TSTA (fraction 15), and intact irradiated MCA-F cells. The TSTA specificity of these xenoantisera suggests that they may provide useful reagents for rapid isolation and characterization of the immunoprotective moiety.
用化学诱导的小鼠肉瘤MCA - F的3M KCl提取物通过制备性等电聚焦部分纯化的免疫保护性肿瘤特异性移植抗原（TSTA）组分免疫的兔子，通过间接膜免疫荧光测定评估产生了特异性异种抗血清。异种抗血清仅对免疫肿瘤MCA - F染色，而不对抗原性不同的MCA - D或MCA - T靶细胞染色。用抗原性不同的MCA - D或MCA - T细胞吸收抗MCA - F抗血清不会降低其与MCA - F细胞结合的能力。免疫荧光反应受到诱导特异性免疫保护的MCA - F组分的竞争性抑制：粗制3M KCl提取物、等电聚焦的TSTA（组分15）和完整的经辐照的MCA - F细胞。这些异种抗血清的TSTA特异性表明它们可能为免疫保护部分的快速分离和表征提供有用的试剂。
