Shimamoto G, Berk R S
Biochim Biophys Acta. 1980 Oct 15;632(3):399-407. doi: 10.1016/0304-4165(80)90235-4.
The metabolic fate of acetate, produced during taurine catabolism in Pseudomonas aeruginosa TAU-5, appears to involve the glyoxylate cycle. Organisms grown on taurine have significantly higher levels of malate synthetase and isocritrate lyase than cells grown on nutrient broth, but were comparable to the levels found in acetate-grown organisms. Itaconate, an isocitrate lyase inhibitor, produced a prolonged lag phase and reduced the growth rate of organisms when it was present in the taurine or acetate growth medium. Ethylmethanesulfonate treatment of TAU-5 yielded mutant strains unable to grow on taurine or acetate as sole carbon sources, due to a lack of either malate synthetase or isocitrate lyase. Spontaneous revertants derived from these mutant strains regained the missing enzyme activity and the ability to grow on taurine or acetate.
在铜绿假单胞菌TAU-5中，牛磺酸分解代谢过程中产生的乙酸盐的代谢命运似乎涉及乙醛酸循环。以牛磺酸为生长底物的生物体中，苹果酸合成酶和异柠檬酸裂解酶的水平显著高于在营养肉汤中生长的细胞，但与在乙酸盐培养基中生长的生物体中的水平相当。异柠檬酸裂解酶抑制剂衣康酸，当存在于牛磺酸或乙酸盐生长培养基中时，会导致生物体出现延长的滞后期并降低其生长速率。用甲基磺酸乙酯处理TAU-5产生了突变菌株，由于缺乏苹果酸合成酶或异柠檬酸裂解酶，这些突变菌株无法以牛磺酸或乙酸盐作为唯一碳源生长。从这些突变菌株衍生出的自发回复突变体恢复了缺失的酶活性以及在牛磺酸或乙酸盐上生长的能力。
