通过电子显微镜研究的假单胞菌细胞色素氧化酶的四级结构。

The quatenary structure of Pseudomonas cytochrome oxidase studied by electron microscopy.

作者信息

Saraste M, Virtanen I, Kuronen T

出版信息

Biochim Biophys Acta. 1977 May 27;492(1):156-62. doi: 10.1016/0005-2795(77)90222-7.

Abstract

Pseudomonas cytochrome oxidase (EC 1.9.3.2) was studied by negative staining in the electron microscope. The best resolution was obtained with uranyl oxalate (pH 6.0) as negative stain. Electron micrographs confirm the idea of the dimeric structure of the enzyme. A rough model of cytochrome oxidase was constructed based on different projections of the molecule seen in the electron micrographs. In this model the subunits are identical and sterically equivalent.

摘要

通过电子显微镜负染色法对铜绿假单胞菌细胞色素氧化酶（EC 1.9.3.2）进行了研究。以草酸铀酰（pH 6.0）作为负染色剂时获得了最佳分辨率。电子显微镜照片证实了该酶二聚体结构的观点。基于在电子显微镜照片中看到的分子不同投影构建了细胞色素氧化酶的粗略模型。在该模型中，亚基是相同的且空间上等效。