Pane G, Wegelin I
Boll Soc Ital Biol Sper. 1980 Feb 29;56(4):385-90.
Glycosaminoglycans extracted by CPC precipitation from chick embryo skin at 9, 12, 15, 18, 21 days of incubation were separated by three different electrophoretic methods on acetate cellulose strips. We observed the presence of Hyaluronic acid, Dermatan Sulfate and Chondroitin-4-Sulfate during the whole period considered and of Heparan Sulfate only after the 9th day. Dermatan Sulfate increases until the 15th day then decreases progressively; on the contrary Hyaluronic acid and Chondroitin-4-Sulfate decrease during days 9 to 15 then increase until hatching. Heparan Sulfate appear the 9th day then increases progressively until hatching.
通过CPC沉淀法从孵化9、12、15、18、21天的鸡胚皮肤中提取的糖胺聚糖,在醋酸纤维素条上通过三种不同的电泳方法进行分离。在整个观察期间,我们都观察到了透明质酸、硫酸皮肤素和硫酸软骨素-4的存在,而硫酸乙酰肝素仅在第9天后出现。硫酸皮肤素在第15天之前增加,然后逐渐减少;相反,透明质酸和硫酸软骨素-4在第9天至第15天减少,然后在孵化前增加。硫酸乙酰肝素在第9天出现,然后逐渐增加直至孵化。