用于气相色谱-质谱联用法定量5-羟基二十碳四烯酸（5-HETE）的十八氘代（±）-5-羟基二十碳四烯酸（5-HETE）的选择性合成。
Selective synthesis of octadeuterated (+/-)-5-HETE for use in GC-MS quantitation of 5-HETE.
作者信息
Hubbard W C, Phillips M A, Taber D F
出版信息
Prostaglandins. 1982 Jan;23(1):61-5. doi: 10.1016/0090-6980(82)90022-3.
5(S)-hydroxy-6 trans-8,11,14 cis-eicosatetraenoic acid (5-HETE) is the major product of arachidonic acid metabolism via the 5-lipoxygenase pathway. A limiting factor in the quantitation of 5-HETE by GC-MS analysis is the availability of a stable isotope analog for use as an internal standard. In this report, we detail procedures for selective chemical synthesis of multimilligram quantities of octadeuterated (+/-)-5-HETE from octadeuterated arachidonic acid. The octadeuterated (+/-)-5-HETE is suitable for use as an internal standard for GC-MS quantitation of 5-HETE. Preparation of the octadeuterated analog of 5-HETE can be readily performed in most laboratory settings.
5(S)-羟基-6反式-8,11,14顺式-二十碳四烯酸（5-HETE）是花生四烯酸经5-脂氧合酶途径代谢的主要产物。通过气相色谱-质谱（GC-MS）分析对5-HETE进行定量的一个限制因素是缺乏用作内标的稳定同位素类似物。在本报告中，我们详细介绍了从十八氘代花生四烯酸选择性化学合成多毫克量的十八氘代(±)-5-HETE的步骤。十八氘代(±)-5-HETE适用于作为GC-MS定量5-HETE的内标。在大多数实验室环境中都可以轻松制备5-HETE的十八氘代类似物。
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