通过对固定化抗体 - 淋球菌酶复合物产生的NADH进行紫外线照射来检测淋病奈瑟菌。
Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex.
作者信息
McDonald H C, Simon P A, Takeguchi M M, Odstrchel G, Weetall H H, Felman Y M
出版信息
Immunol Lett. 1981 Dec;3(6):377-80. doi: 10.1016/0165-2478(81)90070-5.
PMID:6802748
Abstract
Frozen, cervical swabs were placed in a lysis buffer containing an immobilized antibody to the gonococcal enzyme, 1,2-propanediol oxidoreductase. The immobilized antibody--enzyme complex that formed was active after the addition of substrate (1,2-propanediol and NAD) and this activity could be detected by visual inspection of NADH fluorescence under ultraviolet illumination.
摘要
将冷冻的宫颈拭子置于含有固定化抗淋球菌酶（1,2 - 丙二醇氧化还原酶）抗体的裂解缓冲液中。形成的固定化抗体 - 酶复合物在加入底物（1,2 - 丙二醇和NAD）后具有活性，并且这种活性可以通过在紫外光照射下目视检查NADH荧光来检测。
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