Pronin S V, El'-Registan G I, Shevtsov V V, Duda V I
Mikrobiologiia. 1982 Mar-Apr;51(2):314-7.
The dormancy cyst-like forms of Bacillus cereus obtained under the action of an autoregulatory factor were shown to be more resistant to the action of such external factors of the medium as high temperatures, ultraviolet irradiation and low molecular weight alcohols comparing to the vegetative cells. Incubation at 80 degrees C for 15 min killed 98,5% vegetative cells and 86,5% of the dormancy refractile forms. U. V.--radiation treatment resulted in a loss of viability in 97% of the vegetative cells and in 75% of the refractile forms. Differences were also found in the resistance of the cyst-like forms and the vegetative cells to the action of ethanol, methanol and butanol. The resistance of the cyst-like forms of B. cereus to the action of the above damaging factors is consistent, in general, with the resistance of cysts, methylotrophic bacterial spores and other dormancy prokaryotic cells differing in the mode of their formation from endospores.
结果表明,在自调节因子作用下获得的蜡样芽孢杆菌休眠囊样形态比营养细胞对培养基的高温、紫外线照射和低分子量醇类等外部因素的作用具有更强的抵抗力。在80℃下孵育15分钟可杀死98.5%的营养细胞和86.5%的休眠折光形态。紫外线辐射处理导致97%的营养细胞和75%的折光形态丧失活力。在囊样形态和营养细胞对乙醇、甲醇和丁醇作用的抗性方面也发现了差异。蜡样芽孢杆菌囊样形态对上述破坏因素作用的抗性总体上与囊肿、甲基营养细菌孢子和其他休眠原核细胞的抗性一致,它们形成方式与内生孢子不同。