丙卡巴肼治疗小鼠对α-甘油磷酸脱氢酶活性及精子特定异常发生率的影响。

Effect of procarbazine treatment of mice on alpha-glycerolphosphate dehydrogenase activity and frequency of selected abnormalities in sperm.

作者信息

Burkhart J G, Ray C P, Malling H V

出版信息

Mutat Res. 1982 Feb 22;92(1-2):249-56. doi: 10.1016/0027-5107(82)90228-7.

Abstract

The in situ activity of mitochondrial alpha-glycerol phosphate dehydrogenase (alpha-GPD) as well as 2 specific sperm abnormalities [headless and disorganization of the mitochondria assembly (PL-type)] have been studied in sperm from mice treated with single doses of procarbazine (100, 200, 400, 600 and 800 mg/kg) at intervals up to 55 days after treatment. The frequency of sperm without active alpha-GPD and the frequency of the morphology variants increased with increasing dose of procarbazine. The variance in the level of alpha-GPD among sperm with active enzyme and the variance between animals also increased with increasing dose. The lowest effective dose was 200 mg/kg and there were no observable effects 85 days after treatment.

摘要

研究了单剂量丙卡巴肼（100、200、400、600和800mg/kg）处理小鼠后直至处理后55天期间，精子中线粒体α-甘油磷酸脱氢酶（α-GPD）的原位活性以及2种特定的精子异常情况[无头和线粒体组装紊乱（PL型）]。无活性α-GPD的精子频率和形态变异频率随丙卡巴肼剂量增加而升高。有活性酶的精子中α-GPD水平的差异以及动物之间的差异也随剂量增加而增大。最低有效剂量为200mg/kg，处理后85天未观察到明显影响。