Nathrath W B, Wilson P D, Trejdosiewicz L K
Acta Histochem Suppl. 1982;25:73-82.
Antiserum raised in rabbits against the cytosol fraction from calf bladder epithelium was absorbed with sera, human erythrocytes and non-epithelial tissues until it became specific for a number of epithelia including urothelium, as demonstrated by immunofluorescence and immunoperoxidase techniques. Electron microscope immunocytochemistry showed that, in the urothelium, the majority of reaction product was present in the superficial cells in association with the plasma membrane, cytoplasmic membrane vesicles and cytoskeleton. After a second series of absorptions with epithelial organs other than bladder, the antiserum was rendered urothelium-specific and the strongest reaction was seen in superficial layer cells. Epithelium and urothelium specific antigens were species cross-reactive and could also be demonstrated in foetal and malignant urothelium. In malignantly transformed mouse bladder epithelium cell lines, the epithelial antigens were associated with the inner surface of the plasma membrane and underlying cytoskeletal elements.
用兔制备的抗小牛膀胱上皮细胞溶质部分的抗血清，先后用血清、人红细胞和非上皮组织进行吸收处理，直至通过免疫荧光和免疫过氧化物酶技术证明其对包括尿路上皮在内的多种上皮具有特异性。电子显微镜免疫细胞化学显示，在尿路上皮中，大部分反应产物存在于表层细胞中，与质膜、细胞质膜囊泡和细胞骨架相关。在用膀胱以外的上皮器官进行第二轮吸收后，抗血清变得对尿路上皮具有特异性，且在表层细胞中可见最强反应。上皮和尿路上皮特异性抗原具有种间交叉反应性，在胎儿和恶性尿路上皮中也可检测到。在恶性转化的小鼠膀胱上皮细胞系中，上皮抗原与质膜内表面和下面的细胞骨架成分相关。
