Giles A R, Tinlin S, Greenwood R
Blood. 1982 Sep;60(3):727-30.
A model of bleeding due to clotting factor deficiency has been developed in dogs. Normal and hemophilic (factor VIII:C deficient) animals were used. Bleeding was induced in lightly anesthetized animals by severing the apex of the nail cuticle using a guillotine device. In normal animals, bleeding usually ceased spontaneously after 2-8 min. In contrast, in hemophilic animals, bleeding continued for up to 20 min and necessitated either cauterization or the application of topical thrombin to achieve hemostasis. Pretreatment of the hemophilic animals with canine cryoprecipitate corrected the cuticle bleeding time to within the range noted for normal animals. The method is simple and reproducible and has the advantage that a number of observations can be made sequentially on the same animal. Rebleeding of the cauterized cuticle of the hemophilic animals did not usually occur. This model has considerable potential for the preclinical testing of products considered to bypass or replace factor VIII:C in patients with acquired inhibitors of factor VIII:C and may be adapted to the study of other mechanisms involved in normal and abnormal hemostasis.
已在犬类中建立了一种因凝血因子缺乏导致出血的模型。使用了正常动物和血友病(因子VIII:C缺乏)动物。通过使用断头台装置切断甲皱襞顶端,在轻度麻醉的动物中诱导出血。在正常动物中,出血通常在2 - 8分钟后自行停止。相比之下,在血友病动物中,出血持续长达20分钟,需要烧灼或局部应用凝血酶才能实现止血。用犬冷沉淀预处理血友病动物可将甲皱襞出血时间校正到正常动物所记录的范围内。该方法简单且可重复,优点是可以在同一只动物上依次进行多次观察。血友病动物烧灼后的甲皱襞通常不会再次出血。该模型对于在获得性因子VIII:C抑制剂患者中被认为可绕过或替代因子VIII:C的产品的临床前测试具有相当大的潜力,并且可能适用于研究正常和异常止血所涉及的其他机制。